Phosphatidylinositol 3-hydroxy Kinase (PIK3CA) is a kinase that catalyzes the phosphorylation of inositol groups. It is a heterodimer composed of a catalytic subunit and a regulatory subunit, which regulates cell proliferation, adhesion, Survival and motility play an important role in the occurrence and development of tumors. Most of the PIK3CA gene mutations occur in the helical region (exon9) and the kinase region (exon20), and PIK3CA gene mutations have been found in a variety of cancers (such as non-small cell lung cancer, breast cancer, etc.). PIK3CA gene mutation leads to persistent activation of PI3K/Akt signaling pathway, and PI3K is activated as a downstream signaling molecule of EGFR, resulting in tumor cells being resistant to EGFR-TKIs, trastuzumab and other drugs.
This kit is used to detect 5 (H1047R, H1047L, E542K, E545K, E545D) mutation types of the PIK3CA gene, providing a scientific basis for clinicians to select targeted drugs for individualized treatment.